Improved production of alkaline protease from a mutant of alkalophilic Bacillus pantotheneticus using molasses as a substrate

An alkalophilic bacterial isolate identified as Bacillus pantotheneticus, isolated from saline-alkali soils of Avadh region of UP, India, was studied for the production of alkaline protease. The mutant of the isolated species showed 44% improved production over the parent strain. Organic nitrogen sources supported better protease production than the inorganic sources. The production of alkaline protease was (242 U/ml) in the medium containing molasses, which was comparable with molasses and wheat bran (285 U/ml) as carbon and nitrogen sources, respectively. Protease production was best at pH 10 and temperature 30 °C. The Km (for casein) was 11 mg/ml and V max was 380-μg tyrosine/ml/min. The enzyme was stable between pH 7 and 10.7 and temperature between 30 and 60 °C with a pH and temperature optimum at 8.4 and 40 °C, respectively. The results indicated that molasses was an optimal substrate for alkaline protease production.