Detection of a Bacterium Associated with a Leaf Spot Disease of Maize in Brazil

A leaf spot disease of maize occurring in Brazil in the 1980s was described as being caused by the ascomycete Phaeosphaeria maydis (P. Henn) Rane. Payak and Renfro (imperfect form Phyllosticta sp.). Disease symp- toms were dark-green water-soaked spots that later became necrotic lesions. There are no reports at present in the literature of re-infection by the fungus under controlled conditions, casting doubt on the true iden- tity of the pathogen. In this study, cytological analyses of lesions at the initial stages did not detect the presence of fungal structures. Bacterial colonies with yellow pigmentation were isolated from the lesions, which reacted positively in hypersensitivity tests in tobacco plants. Maize plants were inoculated with the isolated bacteria. After 72 h incubation in a dew chamber, plants were transferred to a greenhouse, where they remained until evaluation. Typical symptoms of the disease were observed 5±7 days after inoculation of plants, only on treatments inoculated with the bacteria. The bacterium was re-isolated, which suggests its involvement in the initial phases of disease. The bacterium was identi®ed as Pantoea ananas (synonym Erwinia ananas).