A Simple Technique for Determining the Reaction of Barley Genotypes to Pyrenophora graminea

An in vitro technique was used to determine the reaction of 10barley genotypes to Pyrenophora graminea, the seed-borne pathogen causing barley leaf stripe disease. Determination was based on the percentage of inoculated seeds that produced fungal hyphae when cultured on potato dextrose agar. The technique allows low, intermediate and absolute levels of resistance to leaf stripe to be determined. Genotypes CI-5791 and Banteng were resistant, Thibaut, Igri and PK (30-531) were moderately resistant, Gollf was moderately susceptible, and WI2291, Arabi Abiad, Furat 1 and Arrivate were susceptible. The in vitro and in field assessments were significant (correlation coefficient r ¼ 0.96), results indicating that repeated measurements for infected seeds by this in vitro method were very similar to those of field assessments.