Identification of new allergenic constituents and proof of evidence for coniferyl benzoate in Balsam of Peru

image Balsam of Peru is used worldwide for different purposes. Three quarters of its constituents are still unknown, and its main allergens have not been identified to date. image Chemical examination of balsam of Peru and experimental sensitization was performed to identify the main sensitizers. image Using different analytic methods such as thin-layer chromatography, high-pressure liquid chromatography, gas chromatography/mass spectrometry, and sensitization experiments in guinea pigs resulted in the identification of a greater number of constituents and the determination of their sensitizing potency. image Benzyl isoferulate, coniferyl cinnamate, hydroconiferyl cinnamate, and hydroconiferyl benzoate were identified among others as new constituents. Also, the presence of coniferyl benzoate could be shown for the first time. It showed the strongest sensitizing capacity of all compounds tested followed by benzyl isoferulate. The latter is a significant cross-linking allergen because it occurs in propolis as well. image New sensitizing constituents have been identified in balsam of Peru. Based on these results, a patch test series has been created that is recommended for epicutaneous tests in balsam of Peru-positive patients.