Title of article :
Nitric Oxide Modulation of Neutrophil-Endothelium Interaction: Difference Between Arterial and Venous Coronary Bypass Grafts
Author/Authors :
Massimo Chello MD، نويسنده , , Pasquale Mastroroberto MD، نويسنده , , Francesco Perticone MD، نويسنده , , Vittori Celi MD، نويسنده , , Alfredo Colonn PhD، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1998
Abstract :
Objectives. This study sought to evaluate the relation between the pattern of neutrophil-endothelial adhesion in saphenous vein (SV) and internal mammary artery (IMA) grafts and the endothelial production of nitric oxide (NO).
Background. Autologous IM and SV grafts (SVGs) are increasingly used as conduits for coronary bypass grafting. Previous studies have demonstrated greater production of endothelial-derived relaxing factor (NO) from IM than from SVGs. Because of the well known role of NO in modulating the adhesion of polymorphonuclear leukocytes to the endothelium, we studied the pattern of neutrophil adhesion to the endothelium of IM and SVs under basal conditions and after inhibition of NO synthesis.
Methods. Segments of IM and SVs were obtained from 20 patients undergoing coronary artery bypass graft surgery. We evaluated the adhesion of both unstimulated and activated neutrophils to the endothelial surface of IM and SVs in both basal conditions and after inhibition of NO synthesis with Nω-nitro-image-arginine methyl ester.
Results. Under basal conditions, no difference in unstimulated neutrophil adhesion to endothelium was observed between the two vessel conduits. After neutrophil activation, significantly (p < 0.05) greater adhesion of neutrophils was observed in the SV than in the IMA. After inhibition of NO release, the adhesion of activated neutrophils increased in both vessels, and no significant difference between them was observed. The increased adhesion was attenuated by both image-arginine and sodium nitroprusside.
Conclusions. The lesser neutrophil adhesion to the endothelium of the IM is consequence of enhanced release of NO at this level; this effect could be responsible for the better early and long-term patency of this conduit over the SVG in coronary bypass grafting.
Keywords :
, IMA , internal mammary artery , EDRF , endothelium-derived relaxing factor , Krebs-Henseleit , DiI , dioctadecyloxacarbocyanine perchlorate , K-H
Journal title :
JACC (Journal of the American College of Cardiology)
Journal title :
JACC (Journal of the American College of Cardiology)