Evaluation of a new pretreatment reagent for use with the EMIT®2000 cyclosporine assay

Objective Evaluate the performance of the new pretreatment (NPT) reagent for use with the EMIT® cyclosporine A (CsA) assay. Design and methods Samples from transplant patients receiving CsA were tested using a COBAS MIRA S®. Results A downward shift in target values for commercial controls was observed using the NPT reagent. There was excellent correlation for patient results when comparing the NPT reagent with methanol pretreatment. Conclusion The NPT reagent is an acceptable substitute for methanol in the EMIT® extraction procedure for CsA.