Influence of anticoagulants on the measurement of S100B protein in blood

Objective Evaluate anticoagulants influence on blood S100B levels. Design and methods Blood from 18 healthy adult subjects were collected using: no anticoagulants; EDTA; heparin; and citrate. S100B levels were determined using LIA-mat assay. Results Heparin and citrate increased S100B levels (p< 0.001), whereas EDTA had no effect (p = 0.24). Heparin samples were highly (r2 = 0.97, p< 0.001), citrate samples were moderately (r2 = 0.49, p< 0.001), and EDTA samples were not (r2 = 0.22, p = 0.059) correlated with serum samples. Conclusion When anticoagulant is required, heparin should be the primary choice