Association of IL1B polymorphisms with gastric cancer in a Chinese population

Objectives: To investigate the association between IL1B polymorphisms and risk of gastric cancer in a Chinese population, seven SNPs in the IL1B gene were selected for this study. Methods: A multiplex genotyping method, which is based on adapter ligation and allele-specific amplification, was established to type seven SNPs on the IL1B gene simultaneously. One hundred and forty-one non-cancer outpatients and 97 patients with gastric cancer were genotyped, and the relation between IL1B polymorphisms and gastric cancer was statistically analyzed. Results: The seven SNPs were successfully typed and the results were consistent with those obtained by both Sangerʹs sequencing and PCR-RFLP. Handling with statistical analysis, we observed significantly different genotype frequencies of 0794C > T (χ2 = 6.11, P = 0.05), 1274C > T (χ2 = 6.86, P = 0.03) and 2143T > C (χ2 = 6.86, P=0.03) between patients and controls. Conclusion: ALM-ASA is a potential method for multiplex SNP typing with a low consumption of genomic DNA. SNPs 0794C > T, 1274C > T, and 2143T > C are associated with gastric cancer.