Objectives: Investigation of the effect of hypoxic conditions during labour on the protein oxidative modifications and changes in plasma antioxidative capacity of newborns. Design and methods: Oxidative damage to proteins was determined by high-performanc

Objectives: To develop a kinetic, inexpensive, automatable assay for ceruloplasmin as ferroxidase. Design and methods: Dithiothreitol has been used to stabilize the substrate and quinolones to complex the ferric. Results: Mean ferroxidase activity in healthy adults, Wilsonʹs disease and pregnancy was 787.29, 178.5 and 1828.09 IU/L, respectively. Correlation coefficient of ferroxidase vis-à-vis copper, ferroxidase by ferrozine and ceruloplasmin were 0.90, 0.94 and 0.92, respectively. Conclusions: Norfloxacin method is a simple, inexpensive and automatable kinetic assay.