Performance evaluation of ion exchange and affinity chromatography for HbA1c estimation in diabetic patients with HbD: A study of 129 samples

Objectives To compare ion exchange and boronate affinity chromatography for HbA1c estimation in patients with type I and II diabetes having hemoglobin D. Design and methods Systems based on ion exchange and boronate affinity chromatography were evaluated and compared for their performance for HbA1c estimation in patients with homozygous and heterozygous D disease. Results Boronate affinity chromatography shows least interference by HbD in heterozygous as well as homozygous diabetic patients for HbA1c estimation. Conclusions The use of boronate affinity chromatography was found to be helpful in evaluating glycemic control in diabetic subjects with HbD.