Bioavailability of NO can be estimated by measuring the concentration of nitrate (NO3) in serum. However, the methods used for the measurement NO3 in plasma or serum show a great degree of variation. Therefore, we compared two analytical methods for the

Objectives To identify the molecular lesion in a patient with analbuminemia. Design and methods DNA sequencing, genome-wide SNP microarray and synthetic peptide assays to investigate DNA and protein aberrations. Results A homozygous frameshift deletion in exon 12 of HSA is predicted to cause truncation of the albumin protein. A proalbumin-like sequence identified in the novel C-terminal sequence has the potential to be post-translationally modified. Conclusions The truncated albumin molecule is potentially edited by proteolytic cleavage before it enters the circulation.