Determination of urinary 8-hydroxy-2-deoxyguanosine in obese patients by HPLC with electrochemical detection

A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2ʹdeoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71(plus-minus)7%. Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N=3) was comparable with that achieved using the conventional amperometric detector. Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index>30 kg m^-2) yielding the mean concentration 32(plus-minus)27.1 nM of 8-OHdG or 1.85(plus-minus)0.72 (mu)mol 8-OHdG mol^-1 creatinine (4.63(plus-minus)1.8 (mu)g 8-OHdG g^-1 creatinine), which is not much different from the levels found in healthy individuals.