Author/Authors :
Mete Eray، نويسنده , , Numan S. Dogan and Ercument Arvas ، نويسنده , , Steven R. Reiken، نويسنده , , Himawan Sutisna، نويسنده , , Bernard J. Van Wie، نويسنده , , Alan R. Koch، نويسنده , , David F. Moffett، نويسنده , , Moris Silber، نويسنده , , William C. Davis، نويسنده ,
Abstract :
Methods for developing stable, sensitive and selective bilayer lipid membrane (BLM)-based biosensors are discussed. Stable BLMs were formed over micromachined polyimide apertures. Selective sensors were made by incorporating nicotinic acetylcholine receptors (nAChRs) modified with bispecific antibodies (BsAbs). When two BsAbs, attached to one nAChR, encounter antigen (Ag), channels are blocked. Sensitivity to single Ag molecules would be possible by monitoring closure of individual nAChRs.
Keywords :
Bilayer lipid membranes , Microfabrication , Biosensor , Modified nicotinic acetylcholinereceptor , Bispecific antibodies
