Effects of Lipoxygenase Metabolites of Arachidonic Acid on the Growth of Human Blood CD34+ Progenitors,

The influence of lipoxygenase metabolites of arachidonic acid on proliferation and differentiation of CD34+ cells was studied. Their effects on the CFU-GM and BFU-E progenitors were investigated by culture of CD34+ cells in liquid or semisolid medium. Only 12-HETE (1 μM) stimulated the [3H]thymidine as well as BrdU incorporation and increased the number of cell divisions (PKH2 tracking). Addition of 12-HETE and 15-HETE but not of LXA4, LXB4, LTB4, and LTC4 to liquid cultures of CD34+ cells for 3 and 8 days reduced in a time-dependent manner the number of CFU-GM and BFU-E. Both HETEs also increased the percentage of glycophorin A+ cells while they reduced the percentage of CD34−/CD33+ cells after 3 and 5 days of liquid cultures. These results show that HETE treatment stimulates proliferation and accelerates the differentiation of CD34+ cells, mostly toward the erythroid lineage.