Title of article :
An Efficient Enzymatic Baeyer-Villiger Oxidation by Engineered Escherichia coli Cells under Non-Growing Conditions
Author/Authors :
Walton، Adam Z. نويسنده , , Stewart، Jon D. نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2002
Pages :
-261
From page :
262
To page :
0
Abstract :
Economical methods of supplying NADPH must be developed before biotransformations involving this cofactor can be considered for large-scale applications. We have studied the enzymatic Baeyer—Villiger oxidation ofcyclohexanone as a model for this class of reactions and developed a simple approach that uses whole, non-growing Escherichia coli cells to provide high productivity (0.79 g epsilon-caprolactone/L/h = 18 mumol epsilon-caprolactone/min/g dcw) and an 88% yield. Glucose supplied the reducing equivalents for this process, and no exogenous cofactor was required. The volumetric productivity of non-growing cells was an order of magnitude greater than that achieved with growing cells of the same strain. Cells of an engineered E. coli strain that overexpresses Acinetobacter sp. cyclohexanone monooxygenase were grown under inducing conditions in rich medium until the entry to stationary phase; the subsequent cyclohexanone oxidation was carried out in minimal salts medium lacking a nitrogen source. After the biotransformation was complete, the lactone product was adsorbed to a solid support and recovered by washing with an organic solvent.
Keywords :
cassava blight , phytophatogenic xanthomonads , esterase , bacteriosis
Journal title :
BIOTECHNOLOGY PROGRESS
Serial Year :
2002
Journal title :
BIOTECHNOLOGY PROGRESS
Record number :
5010
Link To Document :
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