Title of article :
Supercritical fluid extraction of the fluoroquinolones norfloxacin and ofloxacin from orally treated-chicken breast muscles
Author/Authors :
KIM، SEON-HYO نويسنده , , Shen، Jing Yu نويسنده , , Kim، Mi Ra نويسنده , , Lee، Chang Joo نويسنده , , Lee، Kang Bong نويسنده , , Shim، Jae Han نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
-450
From page :
451
To page :
0
Abstract :
Supercritical fluid extraction (SFE) of the fluoroquinolones norfloxacin and ofloxacin from chicken breast muscles was examined. A liquid chromatography with fluorescence detection was used for the determination of the fluoroquinolones. Extraction conditions of the SFE were optimized by determining the extraction parameters to achieve a sufficiently high recovery of each fluoroquinolone in fortified-muscle samples. Recovery values for the extraction of the fluoroquinolones using the SFE ranged from 70 to 87%. Chickens were treated orally with each fluoroquinolone and their muscles were extracted at set time intervals for time-course determination of the fluoroquinolones in chickens. The SFE combined with liquid chromatographic analysis showed that the concentrations of the fluoroquinolones decreased gradually with time in the chicken muscles after oral treatment, giving a concentration less than 5 ng/ml in 120 h. No further sample cleanup procedures were required after the SFE. These results suggest that SFE method is an extraction method for the determination of norfloxacin and ofloxacin in chicken muscle.
Keywords :
Norfloxacin , Ofloxacin , Supercritical fluid extraction , SFE , Fluoroquinolones
Journal title :
Analytica Chimica Acta
Serial Year :
2004
Journal title :
Analytica Chimica Acta
Record number :
50316
Link To Document :
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