Quantitative analysis of PPARδ mRNA by real-time RT-PCR in 86 rectal cancer tissues

Aim The purpose of this study is to clarify the expression change of PPARδ gene in human colorectal cancer tissues. Methods Applying real-time RT-PCR, we quantified PPARδ mRNA in a series of 86 tissues from excised primary rectal cancers. In each case, accompanying normal mucosa was collected for comparison. Results Among the 86 rectal cancer tissues, 48 cases showed PPARδ overexpression: 39 tumours gave an expression level 1.5–5 times, five tumours 10–20 times, and four tumours more than 20 times relative to normal mucosa. However, the general level of PPARδ mRNA in rectal cancer tissues is not statistically different from normal mucosa. Conclusions The expression of PPARδ gene in rectal cancers is not statistically different from normal mucosa.