Title of article
Application of Avidin-Biotin Technology and Adsorptive Transfer Stripping Square-Wave Voltammetry for Detection of DNA Hybridization and Avidin in Transgenic Avidin Maize
Author/Authors
Vacek، Jan نويسنده , , Kizek، Rene نويسنده , , Masarik، Michal نويسنده , , Kramer، Karl J. نويسنده , , Billova، Sabina نويسنده , , Brazdova، Marie نويسنده , , Bailey، Michele نويسنده , , Jelen، Frantisek نويسنده , , Howard، John A. نويسنده ,
Issue Information
دوهفته نامه با شماره پیاپی سال 2003
Pages
-2662
From page
2663
To page
0
Abstract
The proteins streptavidin and avidin were electrochemically detected in solution by adsorptive transfer stripping square wave voltammetry (AdTS SWV) at a carbon paste electrode (CPE). AdTS SWV was used to quantify biotinylated oligonucleotides, DNA hybridizations, and avidin in extracts of transgenic avidin maize. The detection limits of denatured and native streptavidin were 6 pM and 120 nM, respectively. The results demonstrated that streptavidin/avidin AdTS SWV is a sensitive and specific method for quantifying DNA and proteins in biological samples such as foods and tissue extracts, including genetically modified crops (avidin maize) and other plants in neighboring fields.
Keywords
Metal-matrix composites (MMCs) , Alloys , Friction/wear , Modelling , Wear coefficient
Journal title
Analytical Chemistry
Serial Year
2003
Journal title
Analytical Chemistry
Record number
51314
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