Title of article :
Transendothelial permeability changes induced by free radicals in an in vitro model of the blood-brain barrier
Author/Authors :
Philippe Lagrange، نويسنده , , Ignacio A. Romero، نويسنده , , Alain Minn، نويسنده , , Patricia A. Revest، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Pages :
6
From page :
667
To page :
672
Abstract :
In the present study, we investigated the changes in blood-brain barrier (BBB) permeability following brain endothelial cell exposure to different xenobiotics able to promote free radical generation during their metabolism. Our in vitro BBB model consisted of confluent monolayers of immortalized rat brain capillary endothelial cells (RBE4) grown on collagen-coated filters in the presence of C6 glioma cells grown in the lower compartment. We have recently shown that a range of xenobiotics, including menadione, nitrofurazone, and methylviologen (paraquat) may undergo monoelectronic redox cycling in isolated brain capillaries, giving rise to reactive oxygen species. In this study, addition of 100 μM menadione to the culture medium for 30 min significantly increased the permeability of endothelial cell monolayers to radiolabeled sucrose. The effect on endothelial permeability induced by menadione was dose-dependent and reversible. These permeability changes preceded the onset of cell death, as assessed by the Trypan blue exclusion method. Pre-incubation with superoxide dismutase and catalase blocked changes in sucrose permeability to control levels in a dose-dependent manner, suggesting the involvement of reactive oxygen species in menadione-induced BBB opening.
Keywords :
Cerebrovascular endothelial cells , free radicals , xenobiotics , oxidative stress , RBE4 cell line , Blood-brain barrier
Journal title :
Free Radical Biology and Medicine
Serial Year :
1999
Journal title :
Free Radical Biology and Medicine
Record number :
518296
Link To Document :
بازگشت