Faster plasma vitamin E disappearance in smokers is normalized by vitamin C supplementation

Vitamin E disappearance is accelerated in cigarette smokers due to their increased oxidative stress and is inversely correlated with plasma vitamin C concentrations. Therefore, we hypothesized that ascorbic acid supplementation (500 mg, twice daily; 2 weeks) would normalize smokersʹ plasma α- and γ-tocopherol disappearance rates and conducted a double-blind, placebo-controlled, randomized crossover investigation in smokers (n = 11) and nonsmokers (n = 13) given a single dose of deuterium-labeled α- and γ-tocopherols (50 mg each d6-RRR-α and d2-RRR-γ-tocopheryl acetate). During the placebo trial, smokers, compared with nonsmokers, had significantly (P < 0.05) greater α- and γ-tocopherol fractional disappearance rates and shorter half-lives. Ascorbic acid supplementation doubled (P < 0.0001) plasma ascorbic acid concentrations in both groups and attenuated smokersʹ, but not nonsmokersʹ, plasma α- and γ-tocopherol (P < 0.05) fractional disappearance rates by 25% and 45%, respectively. Likewise, smokersʹ plasma deuterium-labeled α- and γ-tocopherol concentrations were significantly higher (P < 0.05) at 72 h during ascorbic acid supplementation compared with placebo. Ascorbic acid supplementation did not significantly change (P > 0.05) time of maximal or maximal-labeled α- and γ-tocopherol concentrations. Smokersʹ plasma F2α-isoprostanes were 26% higher than nonsmokers (P > 0.05) and were not affected by ascorbic acid supplementation in either group (P > 0.05). In summary, cigarette smoking increased plasma α- and γ-tocopherol fractional disappearance rates, suggesting that the oxidative stress from smoking oxidizes tocopherols and that plasma ascorbic acid reduces α- and γ-tocopheroxyl radicals to nonoxidized forms, thereby decreasing vitamin E disappearance in humans.