Title of article
Single Pass Sequencing of a Unidirectional Human Fetal Heart cDNA Library to Discover Novel Genes of the Cardiovascular System
Author/Authors
David M. Hwang، نويسنده , , Wei-Sek Hwang، نويسنده , , Choong-Chin Liew، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1994
Pages
5
From page
1329
To page
1333
Abstract
A human fetal heart cDNA library was constructed in the lambda gt22A expression vector. Polymerase chain reaction (PCR) was used to amplify the cDNA inserts. PCR products were purified and used in cycle sequencing reaction in the presence of a fluorescein-conjugated primer and electrophoresed on a Pharmacia A.L.F. Sequencer, Partial cDNA sequences, or expressed sequences tags (ESTs) were searched against the Genbank and EMBI, databases to identify novel genes expresses in the human cardiovascular system
Keywords
Expressed sequence tags (ESTs) , Human Heart cDNA Library
Journal title
Journal of Molecular and Cellular Cardiology
Serial Year
1994
Journal title
Journal of Molecular and Cellular Cardiology
Record number
525194
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