In vivo energy dispersive X-ray fluorescence for measuring the content of essential and toxic trace elements in teeth

The calibration and application of a facility, based on energy dispersive X-ray fluorescent analysis (EDXRF) using 109Cd as an excitation source, for in vivo and in vitro estimation of Ca, Pb, Sr and Zn in tooth enamel is described. During the in vivo measurements, the device ensures tissue protection of face and mouth cavity from radiation, and only a small part of tooth surface under study is irradiated. To calibrate the facility, the contents of Ca, Sr and Zn were analyzed simultaneously in the enamel of 50 teeth by EDXRF and instrumental neutron activation analysis (INAA). Standards prepared from powdered tooth enamel with additions of chemically pure lead compounds were used to calibrate for lead graduation. Enamel calcium is suggested as an internal standard during in vivo EDXRF of teeth. The content of enamel Sr, Zn and Pb was determined by EDXRF in 35 permanent intact teeth of teenagers and adults. It was shown that lead concentration didnʹt exceed 3 μg/g for all the teeth.