Influence of enzymes and plaque acids on in vitro wear of dental composites

The purpose of this investigation was to test the effect of an enzymatic and an acidic medium on in vitro wear of dental resin composites and an amalgam. Wear was evaluated in two-body and three-body wear conditions. The third body was a food slurry either in water, an esterase solution or an acid solution at a composition and concentration as found in caries active plaque fluid. In the two-body tests the slurry was left out. Under three-body wear conditions neither the enzyme nor the acids showed any significant (P < 0.05) effect on wear. Enzyme inactivation from a large excess of food constituents in the slurry or the absence of an effect from surface diffusion rates of the acids resulted in the lack of an effect on wear. The two-body/acids wear rates were statistically (P < 0.05) lower than the two-body/water wear rates caused by increased lubrication from the acids between sliding surfaces. A significant (P < 0.05) increase in wear was found in the two-body/enzyme experiments, showing that the enzymatic activity can start directly at the outer surface of the composites.