Coated reactive carriers based on copolymers of 1-vinyl-2-pyrrolidone and maleic anhydride for immobilization of enzymes

Surface-activated silicas with methacrylate double bonds were introduced into a reaction medium where copolymerization of 1-vinyl-2-pyrrolidone (VP) and maleic anhydride (MA) took place. The copolymerization led to the formation of non-alternating copolymers of VP and MA. Each macromolecule of this copolymer consisted of alternately arranged regulated long sequences of VP units and single units of MA. The possibility for covalent bonding of β-galactosidase to the MA units at different lengths of the VP sequences was studied. The content of coupled enzyme, as well as its activity, pH dependence and stability were determined.