Chemiluminescent properties of some luminol related compounds (II)

Four new luminol related compounds having hydrazino or hydrazono group in the structure, viz. 6-hydrazinophthalazine-1,4(2H,3H)-dione (6-HP), 1,4-dioxo-2,3-dihydrophthalazine-6carbaldehyde hydrazone (6-PCH), 6-hydrazinomethylphthalazine-1,4 (2H,3H)-dione (6HMP) and 6-[N-(3-Propionohydrazino)thioureido]benzo[g]phthalazine-1,4(2H,3H)-dione (PROB), were newly synthesized. The chemiluminescent properties of the compounds were examined, and compared with other luminol analogues. All evaluated compounds produced chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in sodium hydroxide solution. Chemiluminescence intensities were affected by the concentrations of hydrogen peroxide, potassium hexacyanoferrate(III) and sodium hydroxide. Chemiluminescence reaction conditions, and the time dependence of the chemiluminescence of the compounds and luminol as a reference, were studied. 6-HP, 6PCH, 6-HMP and PROB were found to produce chemiluminescence 1.4, 0.2, 0.1 and 32.5% of luminol, respectively.