Cytochrome c from Chromatium vinosum on gold electrodes

Cytochrome cʹ from Chromatium vinosum (CVCP) was immobilized at a surfacemodified gold electrode. Characterization of the CVCP electrode showed a quasireversible, diffusionless electrochemical redox behavior of the surface adsorbed protein with a formal potential of -128±5 mV vs. Ag/AgCl. The heterogeneous electron transfer rate constant of adsorbed CVCP was determined to be about 50 s-1. Different immobilization strategies were compared. The interaction of the immobilized CVCP with nitric oxide (NO) was investigated and applied for a primary amperometric detection of NO in solution.