Title of article :
Dependence of Apparent Viscosity on Mycelial Morphology of Streptomyces fradiae Culture in Various Nitrogen Sources
Author/Authors :
Park، Enoch Y. نويسنده , , Choi، Du Bok نويسنده , , Okabe، Mitsuyasu نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2000
Pages :
-524
From page :
525
To page :
0
Abstract :
To examine what causes increased viscosity in culture broth in Streptomyces fradiae culture, various natural nitrogen sources were investigated. Extracellular protease activity increased with culture time and decomposed the natural nitrogen source into amino acids. In the case of gluten meal, after a culture time of 5 d, concentrations of glutamic acid and aspartic acid had increased to 600 and 200 mg/L, respectively, which were about 3- and 2-fold as high as levels in cultures under similar conditions using Pharmamedia. For various amino acids tested, the addition of glutamic acid or aspartic acid mixture to the culture medium, raised the apparent viscosity to its highest demonstrated value, 260 mPaʹs after 5 d of culture, which was 3-fold higher than without amino acids. Consumption of the decomposed glutamic acid and aspartic acid was dependent on the activities of glutamate dehydrogenase and aspartate aminotransferase, respectively. When ammonium ion was used as the nitrogen source, cell concentration reached 1.75 g/L measured as an intracellular nucleic acid concentration, which was about 2.3-fold higher than that with any other natural nitrogen source. However, apparent viscosity was only 75 mPaʹs, a value one-third that of the amino acid mixture, and 70% of the pellets were bigger than 1.2 x 10* (mu)m2. In the case of gluten meal or the amino acid mixture, pellets bigger than 1.2 x 104(mu)m2 comprised only 8%. This demonstrates that consumption of some amino acids affected the formation of filamentous morphology, which caused an increase in the apparent viscosity of the culture broth, and the apparent viscosity was not caused by the mycelial concentration but the mycelial morphology.
Keywords :
Interference , Time-sharing , Work activity , lnterruptions
Journal title :
BIOTECHNOLOGY PROGRESS
Serial Year :
2000
Journal title :
BIOTECHNOLOGY PROGRESS
Record number :
5507
Link To Document :
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