Title of article
Influence of Baculovirus-Host Cell Interactions on Complex N-Linked Glycosylation of a Recombinant Human Protein
Author/Authors
Shuler، Michael L. نويسنده , , Wood، H. Alan نويسنده , , Joshi، Lokesh نويسنده , , Davis، Thomas R. نويسنده , , Mattu، Taj S. نويسنده , , Rudd، Pauline M. نويسنده , , Dwek، Raymond A. نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
-64
From page
65
To page
0
Abstract
The conditions required for mammalian-type complex N-linked glycosylation of human proteins produced in insect cells with the baculovirus expression vector system were investigated. Marked alterations to N-linked glycosylation of human placental secreted alkaline phosphatase (SEAP) were observed with different baculovirus species, insect cell lines, and cell culture media. When a recombinant Autographa californica nucleopolyhedrovirus (AcMNPV) was used to produce SEAP in Trichoplusia ni (Tn4h) cells cultured in serum-free medium, structural analyses indicated
Keywords
Interference , Work activity , Time-sharing , lnterruptions
Journal title
BIOTECHNOLOGY PROGRESS
Serial Year
2000
Journal title
BIOTECHNOLOGY PROGRESS
Record number
5525
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