Localization and regulation of cytosolic phospholipase A2

Liberation of arachidonic acid by cytosolic phospholipase A2 (cPLA2) upon cell activation is often the initial and rate-limiting step in leukotriene and prostaglandin biosynthesis. This review discusses the essential features of cPLA2isoforms and addresses intriguing insights into the catalytic and regulatory mechanisms. Gene expression, posttranslational modification and subcellular localization can regulate these isoforms. Translocation of cPLA2α from the cytosol to the perinuclear region in response to calcium transients is critical for the immediate arachidonic acid release. Therefore, particular emphasis is placed on the mechanism of the translocation and the role of the proteins and lipids implicated in this process. The regional distribution and cellular localization of cPLA2 may help to better understand its function as an arachidonic acid supplier to downstream enzymes and as a regulator of specific cellular processes.