Probing the Function of Connexin Channels in Primary Tissues

Connexin channels provide for a widespread mechanism of cell-to-cell cross-talk within primary tissues, which is mediated by intercellular exchanges of cytoplasmic ions and molecules. Experimental and clinical studies have recently provided evidence that these exchanges are most likely to play multiple roles, which are critical for the proper development and function of primary tissues. There is also increasing evidence that major clinical disorders may result when the formation and function of connexin channels are altered. Still, the physiological functions that the cell-to-cell communication mediated by connexin channels subserve in most primary tissues are still uncertain. Here, I review two approaches that may aid in identifying these specific functions.