Ue of Topical Human Amniotic Fluid in the Treatment of Acute Ocular Alkali Injurie in Mice Original Reearch Article

Purpoe To evaluate the efficacy of topical human amniotic fluid (HAF) in the treatment of ocular acute alkali burn in mice. Deign Experimental tudy. Method A chemical burn with 2 μl of odium hydroxide 0.15 mol/l wa created in one eye of 30 mice. The animal were divided into gender- and age-matched group according to the topical treatment that wa adminitered: group 1 wa treated with preterm HAF (n = 10 mice); group 2 wa treated with term HAF (n = 10 mice), and group 3 wa treated with aline olution (n = 10 mice). Treatment conited of one drop that wa applied to the burned eye five time per day (week one), and three time per day (week two). The epithelial defect wa photographed and meaured on day two and four. Ocular burn damage wa aeed at day two, even, and 14 after a pre-etablihed claification. On day 14, both eye of each moue were enucleated and aeed hitopathologically. Reult Median epithelial defect (interquartile range [IQR], 25th, 75th percentile) at day four wa 9.93% (IQR, 8.57, 11.27) for group 1, 7.30% (IQR, 5.96, 8.97) for group 2, and 18.92% (IQR, 11.71, 27.64) for group 3 (P < .0076). The overall change (difference in lope) in ocular burn core between day 2 and 14 wa −0.127 (P = .009) in group 1 v 3, −0.134 (P = .012) in group 2 v 3, and 0.007 (P = .88) in group 1 v 2. On hitologic examination aline olution–treated cornea had more inflammatory cell and blood veel than HAF-treated cornea. Concluion Topical preterm/term HAF wa an effective topical therapy for limiting the damage after acute alkali burn of the eye in thi animal model.