In Vitro Evaluation of Combination Antifungal Activity againt Fuarium pecie Iolated from Ocular Tiue of Keratomycoi Patient Original Reearch Article

Purpoe To determine the minimum inhibitory concentration (MIC) of five antifungal agent againt Fuarium pecie iolated from ocular tiue and to evaluate anti-Fuarium pecie activitie of eight combination treatment in vitro. Deign Experimental reearch. Method Thirty-eight iolate of Fuarium pecie were collected from patientʹ ocular tiue and were cultured in vitro. The MIC of natamycin, terbinafine, itraconazole fluconazole, and amphotericin B, either ued alone or combined with other compound, were evaluated by checkerboard microdilution technique baed on the Clinical Laboratory tandard Intitute propoed tandard. The interaction were aeed uing the Fractional Inhibitory Concentration Index model. Reult In the MIC tudy, the MIC90 of each drug ued alone were: natamycin, 16 μg/ml; terbinafine, 8 μg/ml; itraconazole, >16 μg/ml; fluconazole, >64 μg/ml; and amphotericin B, 4 μg/ml. ynergim wa obtained in the amphotericin B plu terbinafine (81.6%) group and in the amphotericin B plu itraconazole (84.2%) group, with an obviouly decreaed MIC value of amphotericin B. Antagonim wa hown in the natamycin plu azole and in the natamycin plu terbinafine group in 52.6% to 60.5% of Fuarium pecie train. Concluion Amphotericin B plu terbinafine or itraconazole demontrated more effective anti-Fuarium pecie activity than ingle-ue in vitro treatment, which implie that thee combination may be helpful in treating fungal keratiti. The combination of natamycin plu azole or natamycin plu terbinafine were not atifactory and can be avoided. Further in vivo tudie are needed to elucidate the potential uefulne of thee combination therapie.