Changes in the size distribution of goat steroidogenic luteal cells during pregnancy

Experiments were conducted to investigate the size distribution of goat steroidogenic luteal cells throughout pregnancy. Corpora lutea were collected from very early (<6 weeks), early (6–8 weeks), middle (9–14 weeks) or late (15–18 weeks) stages of pregnancy. Luteal tissue was dissociated into single-cell suspension by enzyme treatments. Cells were stained for 3betahydroxysteroid dehydrogenase (3beta-HSD) activity, a marker for steroidogenic cells. The steroidogenic cells covered a wide spectrum of size ranging from 5 to 45 ~m in diameter. There was a significant increase in mean cell diameter (P>0.01) as pregnancy progressed. Mean diameter of 3beta-HSD positive cells increased from 14.73±0.35 ~m in the corpus luteum of very early pregnancy to 24.20±0.45 ~m in the corpus luteum of late pregnancy. The ratio of large (>20 ~m in diameter) to small (5–20 ~m in diameter) luteal cells was 0.28:1.0 in very early pregnancy, with the 7.5–15 ~m cell size class being dominant. However, the ratio of large-to-small luteal cells was increased to 1.77:1.0 ~m as pregnancy advanced and 25–35 ~m cell sizes became predominant. It is likely that small luteal cells could develop into large cells as pregnancy progresses. Development of pregnancy is also associated with an increase in size of steroidogenic luteal cells.