Title of article :
Fenofibrate enhances CD36 mediated endocytic uptake and degradation of oxidized low density lipoprotein in adipocytes from hypercholesterolemia rabbit
Author/Authors :
Shui-Ping Zhao، نويسنده , , Jie Wu، نويسنده , , Daqing Zhang، نويسنده , , Hui-Jun Ye، نويسنده , , Ling Liu، نويسنده , , Jie-Qi Li، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
8
From page :
255
To page :
262
Abstract :
CD36 as a fatty acid transporter is predominantly expressed in adipocytes. We studied whether adipocytes could uptake and degrade OxLDL through CD36 and explored the effect of fenofibrate on OxLDL uptake in adipocytes from hypercholesterolemia rabbits. Methods: Subcutaneous adipose tissues were collected from normal, high-cholesterol and high-cholesterol plus fenofibrate treatment rabbits for adipocytes culture. CD36 and peroxisome proliferator-activated receptor γ (PPARγ) mRNA expression were evaluated by RT-PCR. Results : Cellular expression of CD36 was confirmed during differentiation of adipose cell by RT-PCR. Upon incubation at 37 °C, 125I-OxLDL was endocytosed in a dose-dependent fashion and underwent lysosomal degradation by adipocytes. In binding experiments at 4 °C, 125I-OxLDL exhibited specific and saturable binding to adipocytes (KD = 4.2 μg/mL). The endocytic uptake and degradation of 125I-OxLDL by adipocytes were inhibited by 56 and 54% with anti-CD36 antibody. Fenofibrate treatment enhanced the 125I-OxLDL uptake and degradation and up-regulated CD36 mRNA expression in adipocytes and suppressed PPARγ mRNA expression in adipose tissue from hypercholesterolemia rabbits. Conclusion : CD36 plays a novel role in adipose tissues and adipocytes possibly involve in clearance of OxLDL in blood. Fenofibrate treatment improved the OxLDL uptake and degradation in adipocytes from hypercholesterolemia rabbits.
Keywords :
adipocytes , fenofibrate , oxLDL , PPAR? , CD36
Journal title :
Atherosclerosis
Serial Year :
2004
Journal title :
Atherosclerosis
Record number :
631487
Link To Document :
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