Author/Authors :
Sandra Parra، نويسنده , , Carlos Alonso-Villaverde، نويسنده , , Blai Coll، نويسنده , , Natàlia Ferré، نويسنده , , Judit Marsillach، نويسنده , , Gerard Aragonès، نويسنده , , Michael Mackness، نويسنده , , Bharti Mackness، نويسنده , , Lluis Masana، نويسنده , , Jorge Joven، نويسنده , , Jordi Camps، نويسنده ,
Abstract :
Background
Higher high-density lipoprotein concentrations are associated with a better disease course in HIV-infected patients. Paraoxonase-1, an enzyme contained within high-density lipoproteins, is thought to hydrolyse oxidised lipids. The aim of the present study was to investigate the relationships between HIV infection and the circulating activity and concentration of paraoxonase-1, and the concentration of high-density lipoproteins, apolipoprotein A-I and oxidised low-density lipoproteins.
Methods
We studied patients with HIV infection (n = 212) and healthy subjects (n = 409). In all the participants we measured the relevant biochemical and genetic variables. The statistical associations between these variables and paraoxonase-1 activity and concentration were assessed using multiple linear regression analysis.
Results
Serum paraoxonase-1 activity was decreased (P < 0.001) and its concentration was increased (P = 0.017) in HIV-patients compared to the controls. HIV infected patients had lower HDL-cholesterol and apolipoprotein A-I concentrations. Multivariate regression analysis showed that serum paraoxonase-1 activity was associated with the CD4+ T lymphocyte count (P < 0.05), apolipoprotein A-I (P < 0.001), and paraoxonase-1 genetic polymorphisms (P < 0.001). Paraoxonase-1 concentration was associated with that of serum β-2-microglobulin (P < 0.001).
Conclusions
Both, paraoxonase-1 activity and concentration were influenced by HIV-infection and these were related to alterations in HDL composition and the immunological status of the patients.