Objective: Our purpose was to determine whether an abnormal uterine perfusion pattern was associated with subsequent pregnancy loss after fetal cardiac activity was documented. Study Design: Pulsatility indexes of both the right and left uterine arteries

Objective: The study was designed to determine whether moderate chronic hypoxia changes plasma nitrate concentrations in nonpregnant and pregnant, near-term ewes and their fetuses. Study Design: Pregnant ewes were put into either a normoxic control group or a chronically hypoxic group maintained at high altitude (3820 m, PaO2 60 mm Hg) from day 30 of gestation. On day 140 blood samples were collected from the maternal jugular vein and from the fetal umbilical artery and vein. Blood samples were also obtained from normoxic nonpregnant adult ewes and nonpregnant adult ewes exposed to high-altitude hypoxia for 110 days. Plasma nitrate concentrations were determined through chemiluminescence assay. Results: Within normoxic groups plasma nitrate concentrations of pregnant, near-term ewes were significantly elevated compared with those of nonpregnant ewes (17.4 ± 0.3 vs 6.8 ± 0.4 μmol/L, P< .0001). Fetal umbilical arterial and venous plasma nitrate concentrations did not differ from each other in the normoxic group (44.7 ± 4.2 and 44.0 ± 4.3 μmol/L, respectively) but were significantly higher than those of their mothers (P< .0001). Compared with normoxic groups, pregnant ewes with chronic hypoxia showed significantly increased plasma nitrate concentrations (30.9 ± 1.8 μmol/L, P< .001), as did their fetuses (arterial 146.1 ± 14.7 μmol/L, venous 154.5 ± 14.6 μmol/L, P< .0001), but nonpregnant ewes with chronic hypoxia did not (7.5 ± 0.3 μmol/L, P> .05). Conclusions: We conclude that (1) production of endogenous nitric oxide is increased by pregnancy in sheep and is higher in the fetus than in the mother, and (2) moderate chronic hypoxia selectively augments nitric oxide production in the pregnant ewe’s circulation and, even more profoundly, in the fetal lamb’s circulation. (Am J Obstet Gynecol 1998;179:1594-8.)