α1-Adrenergic receptors in cardiac ventricles of dahl rats

This study was designed to examine the effects of sex, age, and a high-salt diet on cardiac α1-adrenoceptors in an animal model of genetic hypertension, the Dahl salt-sensitive rat. Ventricular α1-adrenoceptors were measured by radioligand binding with [3H]prazosin in membrane fractions in Dahl S and R rats of 7, 12, and 15 weeks of age. In both S and R rats, the maximal binding (Bmax) of α1-adrenoceptor binding was greater in male than in female rats. The Bmax decreased with age in both the S and R strains; at 12 weeks of age, Bmax was approximately one-half of that observed at 7 weeks of age in both S and R strains. In the rats fed a high-salt diet, the Bmax tended to be greater in S rats than in R rats at 12 weeks of age and this difference became significant at 15 weeks of age. A significant positive correlation was found between the Bmax and the heart-to-body weight ratio in the Dahl S and R rats. The dissociation constant (Kd) was not different between male S and R rats at each age. These results suggest that the ventricular α1-adrenoceptor may be involved in cardiac hypertrophy in Dahl rats.