A Limited Renal Injury May Cause a Permanent Form of Neurogenic Hypertension

Abstract Previously, we have shown that an acute injury to the kidney produced by an intrarenal injection of phenol causes an immediate increase in blood pressure and in norepinephrine (NE) secretion from the posterior hypothalamus. The studies suggest that in this model afferent impulses from the kidney to central integrative structures in the brain may be responsible for the increase in blood pressure. To further evaluate whether a renal injury caused by the intrarenal injection of phenol leads to a permanent elevation of blood pressure and whether this is mediated by increased sympathetic nervous system activity, we examined the chronic effects (4 weeks) of an intrarenal injection of 50 μL of 10% phenol on blood pressure and NE secretion from the posterior hypothalamus. Systolic blood pressure increased from 128 ± 2.1 to 176 ± 1.5 mm Hg (P< .01) 4 weeks after receiving the intrarenal injection of phenol, but it did not change in rats that received the vehicle (128 ± 2.4 and 135 ± 1.7 mm Hg) and in rats that were subjected to renal denervation (127 ± 3.4 and 124 ± 1.0 mm Hg). The secretion of NE from the posterior hypothalamic nuclei was greater (P< .01) in rats that received phenol (253 ± 9.6 pg/mL) than in controls (158 ± 8.6 pg/mL) and denervated rats (170 ± 2.1 pg/mL). These studies have shown that a limited injury to one kidney may cause a permanent elevation of blood pressure and this is associated with increased sympathetic nervous system activity. Article Outline • Methods • Animals and surgical methods • Microdialysis probe • Location of probe • Norepinephrine microassay • Histologic evaluation • Statistical analysis • Results • Effects of intrarenal injection of phenol on blood pressure and NE secretion from the posterior hypothalamic nuclei • Histologic examination of the injured kidney • Discussion • References