Abstract :
As a newly described member of the apolipoprotein gene family, apolipoprotein A5 (APOA5) has been suggested to play a
key role in the triglyceride metabolism in both human and mice. The aim of this study was to identify the porcine (Sus scrofa)
APOA5 gene, determine its mRNA and its mutations that are associated with lipid accumulation. The porcine APOA5 cDNA was
amplified by reverse transcriptase polymerase chain reaction using the information of the mouse or other mammals. It had
been determined that the open reading frame of the porcine APOA5 gene consists of 1092 bp, which encodes a predicted
protein composed of 363 amino acids with a similarity to bovine (80.43%) and to human (78.47%). The expression analysis
indicated that the porcine APOA5 gene was expressed in hypophysis, fat and liver. Twelve single nucleotide polymorphisms
(SNPs), including 4 SNPs in the 50 end, 1 SNP in second intron, 1 SNP in third exon and 6 SNPs in the 30 end, were identified
in the porcine APOA5 gene and genotyped on the Jinhua3Pietrain F2 reference population, it had revealed that the SNP of
C1834T was significantly associated with average backfat thickness and leaf fat weight ( P,0.01 and P,0.05, respectively).
In conclusion, this study has got basic information of the porcine APOA5 gene and provides evidence that the APOA5 gene
could be a potential candidate gene for fat deposition.
Keywords :
Expression , Polymorphism , APOA5 gene , CLONING , Porcine
