Title of article :
Antioxidant activity, phenol, flavonoid and anthocyanin contents in various extracts of Onosma dichroanthum Boiss. in north of Iran
Author/Authors :
Mazandarani، Masoumeh نويسنده Department of Botany, Islamic Azad University, Gorgan-Branch, Gorgan, Iran , , Zarghami Moghaddam، Parastoo نويسنده Department of Microbiology, Islamic Azad University, Qom- Branch, Qom, Iran , , Baiat، Hooman نويسنده Niak Pharmaceutical Company, Gorgan, Iran , , Zolfaghari، Mohammd Reza نويسنده Department of Microbiology, Islamic Azad University, Qom- Branch, Qom, Iran , , Ghaemi، Ezat Allah نويسنده Department of Microbiology, Infectious Disease Research Center, Golestan University of Medical Sciences, Gorgan, Iran , , Hemati، Helen نويسنده Research Center of Medicinal Pants, Islamic Azad University, Gorgan-Branch, Gorgan, Iran ,
Abstract :
In the present study we carried out a record of the relative antioxidant activity to secondary metabolites content (phenol, flavonoid and anthocyanin) of Onosma dichroanthum Boiss. in various solvent extracts of plant root. The roots were collected in Kiasar Mountain (1800 m) in Mazandaran province, then dried and different extracts were obtained by acetone, ethanol and methanol (absolute and hydro alcoholic), ethyl acetate, chloroform and n-hexane-dichloromethane.The total phenol varied from 4.5 ± 0.7 to 125.6 ± 3.01 mgGAE g -1 in the extracts. Flavonoid contents were between 9.8 ± 3 to 41 ± 2.3 mgQUE g-1, while anthocyanin contents were 11.5± 3.4 to 47.8± 6.8 mgECGgr-1. DPPH (1,1-diphenyl-2-picryl hydrazyl) radical scavenging effect of the extracts was determined spectrophotometrically. The highest radical scavenging effect was observed in acetone extract of plant with EC50 4.06 mg DW. There was a positive correlation between antioxidant activity and total phenolic content for acetone extract. Thus, it was concluded that phenolic compounds were the predominant antioxidant components in the investigated plant species. Therefore, analysis of these results showed acetone was the best solvent to release most secondary metabolites of plant roots, these results will be confirmed the uses of this plant as anti-inflammatory to treat of burn and wounds in their traditional medicine.
