Abstract :
Eglantine rose (Rosa foetida) is an important wild, yellow rose species from Rosaceae family with tiny flowers. It is propagated by such methods as cutting and transplanting. Besides being time-consuming, these methods have usually some problems. Therefore, in the current study the effect of different hormonal concentrations and culture medium on micropropagation of this species was studied in tissue culture medium using axillary shoot explants. The experiment was carried out as a factorial based on a randomized complete with three replications. The results of analysis of variance showed that the number and height of shoots had no significant difference in each explant in two culture media of MS and MS 1.2. However, different concentrations of BA and IBA had significant effect on the number and height of shoots. Means comparison showed that the BA concentration of 4 mg/l with a mean shoot number of 3.75 and IBA concentration of 0.1 mg/l with mean shoot number of 3.166 had the highest effect on shoot number. The BA concentrations of 4, 2 and 6 mg/l with shoot heights of 2.866, 2.833 and 2.375 cm, respectively and IBA concentrations of 0.1, 0.2 and 0 mg/l with shoot heights of 3.06, 2.953 and 2.845 cm, respectively ranked in group A and had the highest shoot height. The BA concentration of 4 mg/l and IBA concentration of 0.1 mg/l had the highest effect on both shoot number and height. The effect of environment on rooting percentage and root number was highly significant. Also, the effect of IAA and IBA hormone on root number was significant. The best culture medium for increasing root number and percentage was found to be MS 1.2 with an IAA concentration of 1.5 mg/l and IBA concentration of 1 mg/l.
