Author/Authors :
Deemeh، Mohammad Reza نويسنده , , Tavalaee، Marziyeh نويسنده , , Ahmadi، Seyed Mahdi نويسنده Isfahan Fertility and Infertility Center, Isfahan, Iran , , Kalantari، Seyed Asadolah نويسنده Isfahan Fertility and Infertility Center, Isfahan, Iran , , Alavi Nasab، Seyed Vali نويسنده Isfahan Fertility and Infertility Center, Isfahan, Iran , , Najafi، Mohammad Hassan نويسنده Isfahan Fertility and Infertility Center, Isfahan, Iran , , NASR ESFAHANI، MOHAMMAD HOSSEIN نويسنده ,
Abstract :
Abstract
This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta)
sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/
in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World
Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were
assessed by chromomycin A3 (CMA3), TUNEL assay and papanicolaou staining, respectively.
Patient was counseled regarding DGC/Zeta sperm preparation procedure. 10 oocytes were injected
with combined DGC/Zeta sperm preparation which resulted in 90% fertilization rate and eight
embryos with good quality. Three embryos were transferred on day three. Singleton pregnancy and
healthy girl baby delivered with cesarean section. Result of this case report opens the horizon for
further evaluation Zeta of sperm selection procedure for couples with repeated ICSI/IVF failure.
