Author/Authors :
Pirestani، Akbar نويسنده , , Hosseini، Sayyed Morteza نويسنده Clinical and Experimental Embryology Department, Cell Sciences Research Centre, Royan Institute (Isfahan Campus), ACECR, Tehran, Iran , , Hajian، Mahdi نويسنده , , Forouzanfar، Mohsen نويسنده , , Moulavi، Fariba نويسنده , , Abedi، Parvaneh نويسنده , , Gourabi، Hamid نويسنده , , Shahverdi، Abdolhossein نويسنده , , Vosough Taqi Dizaj، Ahmad نويسنده Department of Reproductive Imaging, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran , , NASR ESFAHANI، MOHAMMAD HOSSEIN نويسنده ,
Abstract :
Background: The relationship between cyclic status of cattle ovaries on in vitro embryo development
up to the blastocyst stage was investigated.
Materials and Methods: Cattle ovaries were collected immediately after slaughter and divided
into three categories based on their cyclic status, which included: 1. the presence of a large follicle
(LF), 2. the presence of a corpus luteum (CL) and 3. ovaries without LF or CL (WLCF). Oocytes of
these ovaries were obtained and used for in vitro maturation and fertilization. Presumptive zygotes
were then cultured up to the blastocyst stage in synthetic oviductal fluid culture medium.
Results: There were no significant differences between cleavage rates of the three groups. The
rate of embryos in the compact morula stage for the CL group was 48.2% which was significantly
higher than the related rate of the LF group (36.6%), but non-significantly higher than that of
the ST group (45.7%). The highest blastocyst rate belonged to the CL group (54.6%) which was
significantly greater than the WLCF group (32.9%) and non-significantly higher than the LF group
(52.4%). There was no significant difference in blastocyst rates in the CL and LF groups.
Conclusion: Preselection of oocyte donor ovaries containing a CL or LF can be used as a feasible and noninvasive
criterion to obtain the most competent oocytes capable of development to the blastocyst stage.
