Author/Authors :
Vishwanath، نويسنده , , K.، نويسنده , , Ananthararayanan، نويسنده , , T.V.، نويسنده , , Pallavi، نويسنده , , H.M.، نويسنده , , Ramegowda، نويسنده , , Rajendra Prasad، نويسنده , , S.، نويسنده , , Prasanna، نويسنده , , K.P.R. Shailaja Hittalamani، نويسنده ,
Abstract :
Present study characterized 24 popular tomato cultivars using eleven decamer primers. Eleven primers produced 100 bands out of which 89.39 were polymorphic and each cultivar had one or more novel sequences which were not found in other cultivar. These distinguish banding patterns can be successfully used as genetic markers for identification of these cultivars by using specific primer. Primers 0PC-02, OPC-19, OPD-19, OPD-18 and 0PC-08 were found to be the most effective in generating unique bands. All these primers produced total of 13 unique bands in 10 cultivars. It was observed that combination of 0PB-10 either with OPC-19 or 0PB-08 is sufficient to identify all the 24 tomato cultivars in the course of Varietal characterization.
