Induction of Chemosterilization by Single Intratesticular Calcium Chloride Injection in Stray Dogs

The present experiment evaluates the permanent chemosterilizing activity of single intratesticular injection of calcium chloride in stray dog. It has been indicated that testicular seminiferous epithelial germ cell layers were affected drastically along with low sperm count in epididymis, elevation in oxidative stress in testicular tissue, low androgenic enzyme activity, high levels of testicular cholesterol and ascorbic acid after calcium chloride injection. There was no significant change in serum GOT and GPT activities as well as no inflammation was noted in testes. Histoarchitecture of seminiferous tubule at the dose of 200 mg/Kg resulted complete atrophy of germ cells along with infiltration of collagen fibers though the lower dose i.e.100 mg/Kg is not so much effective. There was no change in sex drive or mounting of the treated dog at the dose of 100 mg or 150 mg/Kg body weight, but at the dose of 200 mg/Kg the interference in sex drives with loss of libido was noted. This chemosterilizing ability is permanent as the above mentioned testicular sensors levels remain at the sub fertile range for 60 days when compare to control. This suggests that at a specific dose of single intratesticular injection of calcium chloride is very much effective for induction of chemosterilization.