Title of article :
Identification of Two Types of Growth Hormone Receptor Mutations in Two Strains of Sex-linked Dwarf Chickens
Author/Authors :
Kenichi Tahara، نويسنده , , Akira Tsukada، نويسنده , , Takanobu Hanai، نويسنده , , Kenta Okumura، نويسنده , , Kikumi Yamada، نويسنده , , Atsushi Murai، نويسنده , , Rikiya Yamamoto، نويسنده , , Makoto Maeno، نويسنده , , Noboru Saito and Kiyoshi Shimada، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2009
Abstract :
Sex-linked dwarf (SLD) of White Leghorn (WL; S23MA line, Okazaki station, Japan) and White Plymouth Rock (WPR; 15 line, Hyogo station, Japan) strains were established from the MA line (WL) and 16 line (WPR) of a normal growing line, respectively. However, the responsible genes in the two lines of SLD chicken have not been identified. In this study, we characterized the phenotypes and identified the responsible genes in the SLD chickens of these two strains. SLD chickens of both strains showed low body weight, short tarsometatarsus length, and high blood growth hormone (GH) level compared with the normal growing lines. From these particular results, it was indicated that the SLD chickens might possess a defect in the growth hormone receptor (GHR). We identified two types of mutations in the GHR gene in each SLD chicken by Northern blot and polymerase chain reaction analyses. The S23MA line had a single base mutation in the splice donor site of the exon 5/intron 5 on the GHR gene, whereas the 15 line lacked a large part of exon 10 of the GHR gene, which contained 27 highly conserved amino acids at the 3ʹ end of the coding region and 3ʹ-UTR. Furthermore, it was revealed that growth retardation was caused by reduction in food intake of the SLD chickens. These two genetically distinguishable lines of dwarf chickens would serve as an effective tool for analyzing novel GH function and GHR signal transduction in chickens.
Keywords :
Genotyping , Growth hormone , sex-linked dwarf chicken , growth hormone resistance , Growth hormone receptor
Journal title :
Journal of Poultry Science
Journal title :
Journal of Poultry Science