Determining Protein Patterns for Three Fungus Species Aspergillus fumigatus, Asp. Flavus and Asp. Niger, Obtained from Outdoor Air in Iran

Aspergillus species are saprophytic fungi widely distributed in nature and are associated with a number of human disease. The aim of this study was to compare electrophoretic protein patterns Aspergillus fumigatus, Asp. flavus and Asp.niger and identify the differences of three protein patterns. In this study, three species of Aspergillus fumigatus, Asp.flavus and Asp.niger which were separated from outdoor air in Iran were used to compare electrophoretic protein patterns antigens isolated of Aspergillus. First, these fungi were grown in saboraud glucose agar and preserved at 27°C for 48-72 hours and then they were cultured in saboraud glucose broth to provide protein extract of above mentioned fungi and Bradford method was used in order to measure the level of fungi extracts protein. Protein was dissociated by means of sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) with 11% separating gel. The gel was stained with coomassie brilliant blue G250 and after stabilizing, gel staining and disstaining, different protein bands were appeared. Results revealed, 69 protein bands with molecular weights between 11/5 and 178KD. Among these bands, protein bands with molecular weights of 15, 23/5, 27, 33/5 and 61KD are in two species of Asp.fumigatus and Asp.flavus, protein bands 28/5, 40 and 47KD are in two species of Asp.flavus and Asp.niger, the band with 120 KD is in Asp.fumigatus and Asp.niger and protein bands 23, 35 and 36 KD are among these bands which were presented in every three species of Aspergillus. In addition, a number of strong and weak protein bands were recognized. It was concluded that there was no meaningful relation between protein bands, which were obtained from isolated fungal sp. under study and these three isolates did not follow the same electrophoretic protein patterns.