Biofilm Formation of Escherichia coli Om on Food Contact Stainless Steel and High Density Polyethylene Surfaces

Biofilm have been of considerable interest in the field of food hygiene. Microorganisms attach to surfaces and develop bio films. Biofilm formation by Escherichia coli Om on commonly used surfaces viz stainless steel and high density polyethylene were studied. For this study, 12 stainless steel chips and 12 HDPE chips were used. E.coli strain was added to the beakers with TSB and the samples. E. coli Oin formed biofilm with a mean cell density of 4.14 ± 0.80, 7.69 ±0.19 log CFU/cm2 on stainless steel and HDPE, respectively. There was significant difference (p<0.05) between bacterial counts of two type surfaces. Based on the results, it can be concluded that E. coli Om can survive on food contact surfaces e.g. stainless steel and HDPE surfaces forming biofilm.