Preparation of a Combined Vaccine for Clostridial Diseases and Rabies in Sheep

In the current study, a combined inactivated vaccine was prepared. It was composed of rabies virus and clostridial toxoids (C. perfringens type B and D, C. novyi type B, C. septicum and C. tetani with the whole culture of C. chauvoei) to protect sheep against such fatal diseases which cause high economic losses. After obtaining completely inactivated clostridial toxins and rabies virus using 0.2% BEI, these antigens were mixed together and alhydragel (patent preparation of 2% aluminum hydroxide gel) was added at a rate of 20% in a manner showing that such mixture contains the protective amounts of the including agents. Each vaccine was tested alone in sheep along with combined one to evaluate if there is any interference between these antigens or not. It was indicated that the rabies vaccine did not interfere with the sheep immunity against the clostridial vaccine. On the other hand, the levels of rabies antibodies were increased when clostridial types were added. So that, because of convenience, time consuming and reduction of stress factors by repeat vaccination of single vaccine, it has become better to employ a mixture of such organisms in a single vaccine to be a combined vaccine.