The Effect of CpG ODN on Infectious Bronchitis and Newcastle Disease Vaccines Humoral Immune Response and Phagocytosis in Broilers

Newcastle disease (ND) virus and infectious bronchitis virus (IBV) becomes some of the most important avian infection agents. CpG oligodeoxynucleotides (ODN) are intimated to act as a danger signal to the vertebrate immune system. In present study Chicks were divided into 6 treatment groups; include Normal control, vaccine control, CPG control and 3 different doses of CpG with vaccine. Blood samples were collected at days 7, 14, 21, 28 and 35. IB andND vaccine specific antibody and phagocytosis were evaluated by ELISA and Phagocytosis assay methods, respectively. The comparison of antibody titer and phagocytosis percentage of CpG control and experimental groups with normal control, show significantly (P<0.05, 0.01) higher levels of antibody and phagocytosis in study groups. This Immunostimulating effect of CpG ODN proved to be dose dependent, with better outcome at 100 and 200 fig.